Isolation of human lactate dehydrogenase isoenzyme X by affinity chromatography.

نویسندگان

  • A H Kolk
  • L van Kuyk
  • B Boettcher
چکیده

Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. In the presence of 1.6 mM-NAD+, isoenzyme LDH-X does not bind to AMP-Sepharose, whereas the other lactate dehydrogenase isoenzymes do. This is the crucial point in the isolation of isoenzyme LDH-X from the other isoenzymes. The purified human isoenzyme LDH-X had a specific activity of 146 units/mg of protein.

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عنوان ژورنال:
  • The Biochemical journal

دوره 173 3  شماره 

صفحات  -

تاریخ انتشار 1978